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support@onlinenursepapers.com Kirby Bauer examination (disk diffusion technique)
Antimicrobial Sensitivity Test
The Kirby Bauer examination, also known as the disk diffusion technique, is a commonly used test to determine which antibiotics should be used to treat an infection. This technique relies on measuring the growth of microorganisms under standard conditions.
NURS 6512 Assignment 1: Case Study Assignment: Assessment Tools and Diagnostic Tests in Adults and Children
In this test, a culture medium, specifically Mueller Hinton agar, is carefully and sterilely prepared with the microorganism to be tested. Then, paper disks soaked with a specific concentration of an antibiotic are placed on the agar. The microorganism grows on the agar plate while the antibiotic works to inhibit its growth. If the microorganism is susceptible to a particular antibiotic, no growth will occur around the disk containing that antibiotic.
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Consequently, a zone of inhibition can be observed and measured to determine the microorganism’s susceptibility to the antibiotic. The results are compared to standards set by the Clinical and Laboratory Standards Institute (CLSI). Based on this comparison, the microorganism can be categorized as Resistant (R), Intermediate (I), or Susceptible (S) in the Kirby Bauer examination for antimicrobial sensitivity.
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